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M94A2237.TXT
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1994-10-25
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Document 2237
DOCN M94A2237
TI The anti-HIV activity of recombinant MAP 30 from bitter melon.
DT 9412
AU Lee-Huang S; Bourinbaiar A; Chen HC; Huang P; Huang PL; Kung HF;
Biochem. Dept., NYU Medical School, NY.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):35 (abstract no. 114A). Unique
Identifier : AIDSLINE ICA10/94370338
AB OBJECTIVE: Recently there is an intense community interest in the use of
bitter melon, the source of MAP 30 (Momordinica Anti-HIV Protein, MW
30kD), as an alternative therapy for AIDS. We have cloned and expressed
the gene of this protein. The objective of this study is to determine
the anti-HIV activity of the recombinant MAP 30 (rMAP 30). METHODS: The
antiviral activity of rMAP 30 was studied in acutely as well as
chronically infected cells, by syncytium formation in CEM-ss cells,
viral core protein p24 expression and HIV-reverse transcriptase (RT)
activity in H9 cells. Cytotoxicity was measured by the effects of rMAP
30 on cellular DNA and protein syntheses in uninfected H9 cells.
Toxicity to intact animals was studied on mice. RESULTS: We report here
that rMAP 30 inhibits HIV-1 to the some extent as native MAP 30 (nMAP
30) isolated from the mature bitter melon seeds. In the dose range of
the assay, like its native counterpart, rMAP 30 exhibits little
cytotoxicity in tissue culture or toxicity to intact animals.
Comparisons of the Anti-HIV activity (ID50), cytotoxicity (TD50), and
toxicity (LD50) of rMAP 30 and nMAP 30 are summarized below: TABULAR
DATA, SEE ABSTRACT VOLUME. DISCUSSION AND CONCLUSION: The therapeutic
index of rMAP 30 is comparable to that of nMAP 30, above 10,000. rMAP 30
provides an abundant source of homogeneous material for animal and
pharmacokinetics studies in the development of this anti-HIV agent for
clinical use in patients.
DE Animal Antimetabolites/*PHARMACOLOGY Antiviral Agents/*PHARMACOLOGY
Cells, Cultured Giant Cells/PATHOLOGY Human HIV Core Protein
p24/METABOLISM HIV-1/*DRUG EFFECTS Lectins/PHARMACOLOGY Mice Plant
Proteins/*PHARMACOLOGY/TOXICITY Recombinant Proteins Reverse
Transcriptase/METABOLISM MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).